Structural studies of DNA fragments with base pair mismatches
نویسندگان
چکیده
منابع مشابه
Base-pair mismatches in DNA fragments.
Brutlag, D., Atkinson, M. R., Setlow, P. & Kornberg, A. (1969) Biochem. Biophys. Res. Commun. 37, 982-989 Joyce, C. M. & Grindley, N. D. F. (1983) Proc. Natl. Acad. Sci. U .S .A . 80, 183G1834 Joyce, C. M., Ollis, D. L., Ruch, J., Steitz, T. A,, Knigsber, W. H. & Grindley, N. D. F. (19850) in Protein Structure. Function and Design (Oxender, D. ed.), UCLA Symposia in Molecular and Cellular Biolo...
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Double-stranded DNA may contain mismatched base pairs beyond the Watson-Crick pairs guanine-cytosine and adenine-thymine. Such mismatches bear adverse consequences for human health. We utilize molecular dynamics and metadynamics computer simulations to study the equilibrium structure and dynamics for both matched and mismatched base pairs. We discover significant differences between matched and...
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The response of double-helical DNA to torsional stress may be a driving force for many processes acting on DNA. The 1.55-A crystal structure of a duplex DNA oligonucleotide d(CCAGGCCTGG)(2) with an engineered crosslink in the minor groove between the central guanine bases depicts how the duplex can accommodate such torsional stress. We have captured in the same crystal two rather different conf...
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An assay has been developed that permits analysis of DNA mismatch repair in cell-free extracts of Escherichia coli. The method relies on repair of heteroduplex molecules of f1 R229 DNA, which contain a base-pair mismatch within the single EcoRI site of the molecule. As observed with mismatch heteroduplexes of lambda DNA [Pukkila, P. J., Peterson, J., Herman, G., Modrich, P. & Meselson, M. (1983...
متن کاملRepair of defined single base-pair mismatches in Escherichia coli.
Heteroduplexes with single base-pair mismatches of known sequence were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Each of the eight possible single base-pair mismatches was constructed. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system does not recognize (or...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1987
ISSN: 0108-7673
DOI: 10.1107/s0108767387084496